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 | Acesso ao texto completo restrito à biblioteca da Embrapa Instrumentação. Para informações adicionais entre em contato com cnpdia.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Instrumentação. |
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Data corrente: |
23/06/2021 |
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Data da última atualização: |
10/06/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BUFFO, M. M.; FERREIRA, A. L. Z.; ALMEIDA, R. M. R. G.; FARINAS, C. S.; BADINO, A. C.; XIMENES, E. A.; LADISCH, M. R. |
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Afiliação: |
CRISTIANE SANCHEZ FARINAS, CNPDIA. |
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Título: |
Cellulolytic enzymes production guided by morphology engineering. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Enzyme and Microbial Technology, v. 149, e109833, 2021. |
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Páginas: |
1-8 |
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ISSN: |
0141-0229 |
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DOI: |
https://doi.org/10.1016/j.enzmictec.2021.109833 |
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Idioma: |
Inglês |
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Conteúdo: |
Endoglucanase and xylanase are critical enzymes for liquefaction and enzyme hydrolysis of high solids lignocellulosic biomass to facilitate its transport and production of desired derived products. Here is reported how combinations of different spore concentrations and pH influence microbial morphology, and how this may be used to direct expression and secretion of enzymes by Aspergillus niger. While xylanase production is not affected by A. niger morphology changes, endoglucanase production is enhanced under conditions of lower stress and by morphology that results in pellets. β-glucosidase production is enhanced under dispersed morphology, which results in up to fourfold increase of this enzyme production under the tested experimental conditions. A morphologic scale (Y) is proposed based on a form factor that considers the size and frequency of each morphology class, and that points to conditions that result in high selectivity for either endoglucanase or β-glucosidase production. An equation proposed to relate enzyme activity to morphology provides a useful tool for tuning enzyme production of A. niger, where morphology is a first indication of relative enzyme activities in a fermentation broth. |
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Palavras-Chave: |
(hemi) Cellulases; Filamentous fungi; Morphology engineering. |
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Categoria do assunto: |
-- |
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Marc: |
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024 7 $ahttps://doi.org/10.1016/j.enzmictec.2021.109833$2DOI
100 1 $aBUFFO, M. M.
245 $aCellulolytic enzymes production guided by morphology engineering.$h[electronic resource]
260 $c2021
300 $a1-8
520 $aEndoglucanase and xylanase are critical enzymes for liquefaction and enzyme hydrolysis of high solids lignocellulosic biomass to facilitate its transport and production of desired derived products. Here is reported how combinations of different spore concentrations and pH influence microbial morphology, and how this may be used to direct expression and secretion of enzymes by Aspergillus niger. While xylanase production is not affected by A. niger morphology changes, endoglucanase production is enhanced under conditions of lower stress and by morphology that results in pellets. β-glucosidase production is enhanced under dispersed morphology, which results in up to fourfold increase of this enzyme production under the tested experimental conditions. A morphologic scale (Y) is proposed based on a form factor that considers the size and frequency of each morphology class, and that points to conditions that result in high selectivity for either endoglucanase or β-glucosidase production. An equation proposed to relate enzyme activity to morphology provides a useful tool for tuning enzyme production of A. niger, where morphology is a first indication of relative enzyme activities in a fermentation broth.
653 $a(hemi) Cellulases
653 $aFilamentous fungi
653 $aMorphology engineering
700 1 $aFERREIRA, A. L. Z.
700 1 $aALMEIDA, R. M. R. G.
700 1 $aFARINAS, C. S.
700 1 $aBADINO, A. C.
700 1 $aXIMENES, E. A.
700 1 $aLADISCH, M. R.
773 $tEnzyme and Microbial Technology$gv. 149, e109833, 2021.
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Embrapa Instrumentação (CNPDIA) |
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| Registros recuperados : 15 | |
| 6. |  | LADISCH, M. R.; XIMENES, E.; FARINAS, C. S.; KIM, Y.; KO, J. K.; KREKE, T. Mechanisms of Lignin Derived Inhibition in Hydrolysis of Pretreated Biomass at Low Enzyme Loadings. In: SYMPOSIUM ON BIOTECHNOLOGY FOR FUELS AND CHEMICALS, 38., 2016, Baltimore. Papers. [S. l.: s. n.], 2016. P. 12-1.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Instrumentação. |
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| 7. | | CUNHA, F. M.; BADINO, A.; FARINAS, C. S.; XIMENES, E.; LADISCH, M. R. Liquid hot water and steam explosion pretreatment of sugarcane bagasse for enzyme production by a sequential solid-state and submerged method. In: CONGRESSO BRASILEIRO DE ENGENHARIA QUÍMICA - COBEQ, 20.; ENCONTRO BRASILEIRO SOBRE O ENSINO DE ENGENHARIA QUÍMICA, 15., 2014, Florianópolis. Anais... Florianópolis: UFSC, 2014. Não paginado.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Instrumentação. |
|  |
| 8. | | CUNHA, F. M.; BADINO, A. C.; FARINAS, C. S.; KREKE, T.; XIMENES, E.; LADISCH, M. Liquefaction of sugarcane bagasse for enzyme production. In: SYMPOSIUM ON BIOTECHNOLOGY FOR FUELS AND CHEMICALS, 36., 2014, Clearwater Beach, FL. Anais... Fairfax: Society for Industrial Microbiology and Biotechnology, 2014. p. 84| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Instrumentação. |
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| 9. | | CUNHA, F. M. KREKE, T. BADINO, A. C.; FARINAS, C. S.; XIMENES, E. LADISCH, M. R. Liquefaction of sugarcane bagasse for enzyme production. Bioresource Technology, Essex, v. 172, p. 249-252, 2014.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Instrumentação. |
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| 10. | | FLORENCIO, C.; CUNHA, F. M.; BADINO, A. C.; FARINAS, C. S.; XIMENES, E.; LADISCH, M. R. Secretome analysis of Trichoderma reesei and Aspergillus nigercultivated by submerged and sequential fermentation processes:Enzyme production for sugarcane bagasse hydrolysis. Enzyme and Microbial Technology, [S. l.], v. 90, p. 53-60, 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Instrumentação. |
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| 11. | | FLORENCIO, C.; CUNHA, F. M.; BADINO, A. C.; FARINAS, C. S.; XIMENES, E.; LADISCH, M. R. Secretome data from Trichoderma reesei and Aspergillus niger cultivated in submerged and sequential fermentation methods. Data in Brief, [S. l.], v. 8, p. 588-598, 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 4 |
| Biblioteca(s): Embrapa Instrumentação. |
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| 12. | | BUFFO, M. M.; FERREIRA, A. L. Z.; ALMEIDA, R. M. R. G.; FARINAS, C. S.; BADINO, A. C.; XIMENES, E. A.; LADISCH, M. R. Cellulolytic enzymes production guided by morphology engineering. Enzyme and Microbial Technology, v. 149, e109833, 2021. 1-8| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Instrumentação. |
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| 13. | | HENGGE, N.; KIM, D.; MOSIER, N. S.; KIM, Y.; XIMENES, E.; LADISCH, M. R.; CUNHA, F.; BADINO, A. C.; FARINAS, C. S. Liquefaction of lignocellulose. In: AICHE ANNUAL MEETING, 2016. Abstracts... [S. l.: s. n.], 2016. n. 474787.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Instrumentação. |
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| 15. | | SERRA, L. A.; CRUZ, R. G. da S.; GUTIERREZ, D. M. R.; CRUZ, A. J. G.; CANIZARES, C. A. T.; CHEN, X.; MOSIER, N.; THOMPSON, D.; ASTON, J.; DOOLEY, J.; SHARMA, P.; DE MARCO, J. L.; ALMEIDA, J. R. M. de; ERK, K.; XIMENES, E.; LADISCH, M. R. Screening method for Enzyme-based liquefaction of corn stover pellets at high solids. Bioresource Technology, v. 363, 127999, Nov., 2022.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Agroenergia. |
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| Registros recuperados : 15 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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